Journal: Biochemical and biophysical research communications

Article Title: Histone deacetylase 8 inhibition suppresses mantle cell lymphoma viability while preserving natural killer cell function

doi: 10.1016/j.bbrc.2020.11.001

Figure Lengend Snippet: (A) Transcriptional expression of HDACs during cytokine stimulation of primary human NK cells. RNA from freshly isolated human NK cells or NK cells stimulated with IL2, IL12, and IL18 for 24 hours were assessed by RT-qPCR. The data represents the average of 3 independent experiments with the error bars representing the standard deviation. *p<0.05. (B) Immunoblot of HDAC8 protein expression in primary NK and NK cell lines as indicated. (C) Viability of primary NK cells during HDAC8 inhibition. NK cells were treated with PCI-34051, vorinostat, or LBH-589 at the indicated concentration, followed by stimulation with IL2, IL12, and IL15. Viability was assessed by CTG at the times indicated and normalized to the vehicle (DMSO) control. Data represents the average of 3 independent experiments and error bars represent the standard deviation. (D) Viability of ex vivo expanded NK cells during HDAC8 inhibition. Lymphokine activated killer cells were expanded for 9 days, rested (100U/ml IL2) or subsequently reactivated (500U/ml IL2) overnight, followed by exposure to PCI-34051 at the indicated concentration. Viability was assessed as in panel C. Data represents the average of 2 independent experiments with the error bars representing the standard deviation.

Article Snippet: Lymphokine activated killer cells were generated per manufactures protocol (#130-094-483, Miltenyl Biotec) and expanded for 9 days with 500U/mL IL2.

Techniques: Expressing, Isolation, Quantitative RT-PCR, Standard Deviation, Western Blot, Inhibition, Concentration Assay, Control, Ex Vivo

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Cancer-associated fibroblasts promote pro-tumor functions of neutrophils in pancreatic cancer via IL-8: potential suppression by pirfenidone

doi: 10.1007/s00262-025-03946-z

Figure Lengend Snippet: IL-8 secreted by activated neutrophils is suppressed by PFD. A IL-8 secretion by neutrophils measured by ELISA. IL-8 secretion was significantly increased in neutrophils stimulated with CAF-CM compared to non-stimulated neutrophils. PFD administration during neutrophil activation with CAF-CM significantly suppressed IL-8 secretion. B Effect of secretions from activated neutrophils on cancer cell proliferation. The presence of neutrophils significantly increased MIA PaCa-2 proliferation. Mean ± SD is shown. Each dot represents the number of cells per well. Student’s t-test was used. ***, P < 0.001. C Pancreatic cancer cells induce fibroblasts to become iCAFs, and iCAF-CM activates neutrophils, increasing their IL-8 secretion and enhancing the malignant traits of pancreatic cancer cells. Conversely, PFD acts on activated neutrophils, suppressing the malignant traits of pancreatic cancer cells. This figure was created with BioRender.com

Article Snippet: To evaluate IL-8 levels in neutrophil secretions, culture supernatants were assayed using a human IL-8 enzyme-linked immunosorbent assay (ELISA) kit (Proteintech, KE00006) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Activation Assay

Journal: BMC cancer

Article Title: Exercise accelerates recruitment of CD8 + T cell to promotes anti-tumor immunity in lung cancer via epinephrine.

doi: 10.1186/s12885-024-12224-7

Figure Lengend Snippet: Fig. 3 Transcriptional targets were examined by RNA-seq in tumors after exercise. a The number of differentially expressed genes in the Ex and Ctrl mice were detected by RNA-seq. b Volcano plot of differentially expressed genes in Ex compared with Ctrl tumor tissues. Red dots represent upregu lated genes, and blue dots represent downregulated genes. c Heat map of the differentially expressed genes in Ex compared with Ctrl tumor tissues. d Pathway enrichment analysis of significantly upregulated or downregulated genes. e-j RT-qPCR validates the gene expression of Cxcl10, Cxcl12, Cxcl14, Ppbp, Pf4 and Ccl8 in the tumors each group (n = 3) after tumor inoculation. k-l The expression levels of Cxcl10 and Cxcl12 were detected in the tumors in each group by ELISA (n = 8). The results of e-l are presented as mean ± SEM. Statistical analysis was performed using two-tailed unpaired t tests. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The concentrations of Ccl5, Cxcl10 and Cxcl12 were measured in Lewis lung cancercellsculture supernatants and in the serum of mice using Ccl5 ELISA kit (Cusabio, CSBE09256m), Cxcl10 ELISA kit (Cusabio, CSB-E08183m) and Cxcl12 (Cusabio, CSB-E04723m).

Techniques: RNA Sequencing, Quantitative RT-PCR, Gene Expression, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: BMC cancer

Article Title: Exercise accelerates recruitment of CD8 + T cell to promotes anti-tumor immunity in lung cancer via epinephrine.

doi: 10.1186/s12885-024-12224-7

Figure Lengend Snippet: Fig. 4 Exercise promotes CD8+ T cell recruitment with the assistance of Ccl5 and Cxcl10. a-d Correlation between Ccl5, Cxcl9, Cxcl10, and Cxcl11 mRNA level and CD8+ T cell using QUANTISEQ. Data were obtained from TIMER 2.0 (http://timer.comp-genomics.org/). e-h RT-qPCR analyses of mRNA expres sion of chemokines in the tumors of Ctrl and Ex groups (n = 3). i-j ELISA analysis of serum levels of Cxcl10 and Ccl5 in Ctrl and Ex mice (n = 8). k-m RT-qPCR analyses of mRNA expression of cytokines and PD-L1 in the tumors of Ctrl and Ex mice (n = 3). n Western blot analysis the expression of PD-L1 in the tu mors of Ctrl and Ex mice (n = 3). Full-length blots/gels are presented in Fig. 4n of source data. o Automatic quantification of PD-L1 IHC staining in indicated mice tumors (n = 8). p IHC staining of PD-L1 on tumor sections from different groups (n = 8). q Western blot analysis the expression of P53 in the tumors of Ctrl and Ex mice (n = 3). Full-length blots/gels are presented in Fig. 4q of source data. The results of e-m and o are presented as mean ± SEM. Statistical analysis was performed using two-tailed unpaired t tests. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The concentrations of Ccl5, Cxcl10 and Cxcl12 were measured in Lewis lung cancercellsculture supernatants and in the serum of mice using Ccl5 ELISA kit (Cusabio, CSBE09256m), Cxcl10 ELISA kit (Cusabio, CSB-E08183m) and Cxcl12 (Cusabio, CSB-E04723m).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Immunohistochemistry, Two Tailed Test

Journal: BMC cancer

Article Title: Exercise accelerates recruitment of CD8 + T cell to promotes anti-tumor immunity in lung cancer via epinephrine.

doi: 10.1186/s12885-024-12224-7

Figure Lengend Snippet: Fig. 5 Exercise inhibits tumor progression by upregulation of EPI levels. a Serum levels of EPI in Ctrl, Ex and Ex-post groups were detected by ELISA in tumor-bearing mice (n = 8). b-d EPI mice receiving daily injections of EPI (0.5 mg/kg i.p.) (n = 8) and TE mice with daily running for 14 days after tumor inoculation (n = 8) compared with TC mice (n = 8). b Representative images. c Tumor weights of indicated groups. d Tumor volumes of different groups. e IHC staining of CD8, CD4, CD3 and GZMB on tumor sections from indicated groups (n = 8). f Automatic quantification of CD8, CD4, CD3 and GZMB IHC staining in indicated mice tumors (n = 8). Data are presented as the mean ± SEM in each group. Statistical analysis of a was performed using two-tailed unpaired t tests. c, d and f were performed using one-way analysis of variance (ANOVA). *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The concentrations of Ccl5, Cxcl10 and Cxcl12 were measured in Lewis lung cancercellsculture supernatants and in the serum of mice using Ccl5 ELISA kit (Cusabio, CSBE09256m), Cxcl10 ELISA kit (Cusabio, CSB-E08183m) and Cxcl12 (Cusabio, CSB-E04723m).

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Two Tailed Test